Cover image for DNA science : a first course
Title:
DNA science : a first course
Author:
Micklos, David A.
Personal Author:
Edition:
Second edition.
Publication Information:
Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press, [2003]

©2003
Physical Description:
xii, 575 pages : illustrations (some color) ; 29 cm
Language:
English
ISBN:
9780879696368
Format :
Book

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Summary

Summary

The increased military employment of remotely operated aerial vehicles, also known as drones, has raised a wide variety of important ethical questions, concerns, and challenges. Many of these have not yet received the serious scholarly examination such worries rightly demand. This volume

attempts to fill that gap through sustained analysis of a wide range of specific moral issues that arise from this new form of killing by remote control. Many, for example, are troubled by the impact thatkilling through the mediated mechanisms of a drone half a world away has on the pilots who fly

them. What happens to concepts such as bravery and courage when a war-fighter controlling a drone is never exposed to any physical danger?



This dramatic shift in risk also creates conditions of extreme asymmetry between those who wage war and those they fight. What are the moral implications of such asymmetry on the military that employs such drones and the broader questions for war and a hope for peace in the world going forward? How

does this technology impact the likely successes of counter-insurgency operations or humanitarian interventions? Does not such weaponry run the risk of making war too easy to wage and tempt policy makers into killing when other more difficult means should be undertaken?



Killing By Remote Control directly engages all of these issues. Some essays discuss the just war tradition and explore whether the rise of drones necessitates a shift in the ways we think about the ethics of war in the broadest sense. Others scrutinize more specific uses of drones, such as their

present use in what are known as "targeted killing" by the United States. The book similarly tackles the looming prospect of autonomous drones and the many serious moral misgivings such a future portends.


Author Notes

David A. Crotty: Cold Spring Harbor Laboratory
David Micklos is Executive Director of the Dolan DNA Learning Center at Cold Spring Harbor Laboratory
Greg Freyer is Associate Professor of Clinical Environmental Health Sciences and Anatomy and Cell Biology at Columbia University College of Physicians & Surgeons


Table of Contents

Dedicationp. v
Prefacep. ix
Acknowledgmentsp. xi
Chapter 1 How We Learned That DNA Is the Genetic Materialp. 3
Chapter 2 How We Learned the Function of DNAp. 37
Chapter 3 How We Learned How Genes Are Regulatedp. 69
Chapter 4 Basic Tools and Techniques of DNA Sciencep. 107
Chapter 5 Methods for Finding and Expressing Important Genesp. 141
Chapter 6 Modern Methods for Analyzing Whole Genomesp. 183
Chapter 7 The DNA Science of Cancerp. 221
Chapter 8 Applying DNA Science to Human Genetics and Evolutionp. 259
Laboratories: Lab Safety and Adherence to National Institutes of Health Guidelinesp. 315
1 Measurements, Micropipetting, and Sterile Techniquesp. 321
2 Bacterial Culture Techniquesp. 331
A. Isolation of Individuals Coloniesp. 333
B. Overnight Suspension Culturep. 340
C. Mid-log Suspension Culturep. 345
3 DNA Restriction Analysisp. 351
4 Effects of DNA Methylation on Restrictionp. 375
5 Rapid Colony Transformation of E. coli with Plasmid DNAp. 385
6 Assay for an Antibiotic Resistance Enzymep. 399
7 Purification and Identification of Recombinant GFPp. 411
A. Purification of GFP by HICp. 412
B. PAGE Analysis of Purified GFPp. 417
8 Purification and Identification of Plasmid DNAp. 423
A. Plasmid Minipreparation of pAMPp. 424
B. Restriction Analysis of Purified pAMPp. 431
9 Recombination of Antibiotic Resistance Genesp. 443
A. Restriction Digest of Plasmids pAMP and pKANp. 445
B. Ligation of pAMP and pKAN Restriction Fragmentsp. 452
10 Transformation of E. coli with Recombinant DNAp. 457
A. Classic Procedure for Preparing Competent Cellsp. 458
B. Transformation of E. coli with Recombinant DNAp. 463
11 Replica Plating to Identify Mixed E. coli Populationsp. 473
12 Purification and Identification of Recombinant DNAp. 481
A. Plasmid Minipreparation of pAMP/pKAN Recombinantsp. 482
B. Restriction Analysis of Purified Recombinant DNAp. 487
Answers to Discussion Questionsp. 501
Appendices
1 Equipment, Supplies, and Reagentsp. 521
2 Recipes for Media, Reagents, and Stock Solutionsp. 529
3 Restriction Map Data for pAMP, pKAN, pBLU, pGREEN, and Bacteriophage [lambda]p. 547
4 Cautionsp. 563
Name Indexp. 559
Subject Indexp. 561