Cover image for Spectrophotometry and spectrofluorimetry : a practical approach
Title:
Spectrophotometry and spectrofluorimetry : a practical approach
Author:
Gore, Michael G.
Publication Information:
Oxford ; New York : Oxford University Press, 2000.
Physical Description:
xvii, 368 pages : illustrations ; 26 cm.
Language:
English
Contents:
Introduction to light absorption : visible and ultraviolet spectra / Robert K. Poole and Uldis Kalnenieks -- Fluorescence principles and measurement / Arthur G. Szabo -- Time-resolved fluorescence and phosphorescence spectroscopy / Thomas D. Bradrick and Jorge E. Churchich -- Introduction to circular dichroism / Alison Rodger and Matthew A. Ismail -- Quantitative determination of equilibrium binding isotherms for multiple ligand-macromolecule interactions using spectroscopic methods / Wlodzimierz Bujalowski and Maria J. Jezewska -- Steady-state kinetics / Athel Cornish-Bowden --Spectrophotometric assays / T.J. Mantle and D.A. Harris -- Stopped-flow spectroscopy / M.T. Wilson and J. Torres -- Stopped-flow fluorescence spectroscopy / Michael G. Gore and Stephen P. Bottomley -- Stopped-flow circular dichroism / Alison Rodger and Michael J. Carey -- Spectrophotometry and fluorimetry of cellular compartments and intracellular processes / C. Lindsay Bashford -- Use of optical spectroscopic methods to study the thermodynamic stability of proteins / Maurice R. Eftink and Haripada Maity -- The use of spectroscopic techniques in the study of DNA stability / John SantaLucia, Jr.
Added Author:
ISBN:
9780199638130

9780199638123
Format :
Book

Available:*

Library
Call Number
Material Type
Home Location
Status
Central Library QP519.9.S58 S643 2000 Adult Non-Fiction Central Closed Stacks
Searching...

On Order

Summary

Summary

This book helps readers better understand the background concepts and practical application of spectrophotometry and spectrofluorimetry. Optical spectroscopy underpins the day-to-day operations of most laboratories in the chemical, biological, and medical sciences. This edition contains substantially updated chapters addressing the principles of most of the more common applications, such as: spectrophotometry, spectrophotometric assays, spectrofluorimetry, time resolved fluorescence and phosphorescence studies, circular dichroism, and pre-equilibrium spectroscopic techniques. In all chapters, the emphasis is placed upon the practical aspects, with protocols to guide readers through test experiments. Several chapters introduce subjects that have traditionally depended upon spectroscopy, such as basic enzyme kinetics, ligand binding, data handling, and the more recent interest in the study of protein and DNA stability. Finally, the concept of 'global analysis' is introduced to provide the reader with an insight into this method of utilizing the vast arrays of experimental data provided by current instrumentation.


Table of Contents

List of protocols
Abbreviations
1 Introduction to light absorption visible and ultraviolet spectraRobert K. Poole and Uldis Kalnenieks
2 Fluorescence principles and measurementArthur G. Szabo
3 Time resolved fluorescence and phosphorescence spectroscopyThomas D. Bradrick and Jorge E. Churchich
4 An introduction to circular dichroismAlison Rodger and Matthew A. Ismail
5 Quantitative determination of equilibrium binding isotherms for the multiple ligand - macromolecule interactions using spectroscopic methodsWlodzimierz Bujalowski and Maria J. Jezewska
6 Steady state kineticsAthel Cornish-Bowden
7 Spectrophotometric assaysT.J. Mantle and D.A. Harris
8 Stopped flow spectroscopyM.T. Wilson and J. Torres
9 Stopped flow fluorescence spectroscopyMichael G. Gore and Stephen P. Bottomley
10 Stopped flow circular dichroismAlison Rodger and Michael J. Carey
11 Spectrophotometry and fluorimetry of cellular compartments and intracellular processesC. Lindsay Bashford
12 Use of optical spectroscopic methods to study the thermodynamic stability of proteinsMaurice R. Eftink and Haripada Maity
13 The use of spectroscopic techniques in the study of DNA stabilityJohn SantaLucia, Jr.
List of suppliers
Index

Google Preview